Tau-GFP genetically modified mouse embryonic fibroblasts (Stanford University)

After the head and the vertebral tissues of the embryos were removed, the remaining tissues were minced and digested in trypsin (2.5 mg/ml) to create a single-cell suspension. The cells were plated on tissue-culture dishes and cultured in mouse embryonic fibroblast (MEF) medium consisting of Dulbecco’s modified Eagle’s medium (DMEM) containing fetal bovine serum (FBS, 10%), β-mercaptoethanol, non-essential amino acids, sodium pyruvate and penicillin/streptomycin.