Generation of hematopoietic progenitor cells, megakaryocytes and platelets, using feeder cells
This protocol describes a method to generate megakaryocytes and platelets via the formation of ES-sacs.
These ES-sacs consisted of multiple cysts and spherical cells expressing hematopoietic markers.
The number of ES-sacs generated
from KhES-3 was several-fold higher than those from KhES-1 or
KhES-2.
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Summary for
Generation of hematopoietic progenitor cells, megakaryocytes and platelets, using feeder cells