Generation of bone tissue constructs from pluripotent stem cells

This protocol describes a method to generate bone-like constructs from pluripotent stem cells. Cells differentiate into mesenchymal progenitor cells, and are then expanded. Then, the mesenchymal progenitor cells are seeded onto decellularized bone scaffolds and are cultured first on 2D plates and then in a perfusion bioreactor for several weeks, to form bone-like constructs. These constructs exhibit bone tissue morphology and stain positive for bone markers. Subcutaneous implantation into immunodeficient mice did not result in teratoma formation, and the tissue maintained its bone-like properties.