Generation of hepatocyte-like cells from human pluripotent stem cells
This protocol describes a method to generate hepatocyte-like cells from human pluripotent stem cells. The differentiation process includes high-efficiency differentiation into definitive endoderm-like cells, followed by hepatic specification towards the generation of hepatocyte-like cells. These cells are characterized both by validation assays and by a microarray experiment.
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Generation of hepatocyte-like cells from human pluripotent stem cells