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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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LifeMap Discovery

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All in Vitro Cells > Induced Pluripotent Stem Cell card

178B5 (RIKKEN BioResource Center)

A line of mouse induced pluripotent stem cell (iPS) was established from embryonic fibroblasts by transduction of three factors (OCT3/4, SOX2 and KLF4), using a retrovirus vector. The cells express GFP under the NANOG promoter (Nanog-GFP-IRES-Puro^r).


The cells are cultured on feeder cells (irradiated SNL 76/7) in DMEM (high glucose, without sodium pyruvate), supplemented with FBS (15%), NEAA (0.1mM), 2-mercaptoethanol (0.1mM) and mouse LIF (1000U/ml).

See additional Stem, Progenitor & Primary Cells for: Inner Cell Mass
Induced Pluripotent Stem Cell
Mouse
178B5