Bone marrow-derived mononuclear cells (Franziskus-Krankenhaus)

450–500 ml bone marrow was aspirated from the iliac crest and anticoagulated with unfractionated 100 U/ml heparin. Cells were separated by Ficoll-Paque density gradient centrifugation. The resulting BM-MNC-rich pellet was resuspended and washed three times in isotonic saline. The pellet was resuspended in Ringer’s lactate solution to a final volume of 55–85 ml.