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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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All in Vitro Cells > Fetal Stem / Progenitor Cell card

Umbilical cord blood-derived mesenchymal stem cells (Cedars-Sinai Medical Center)

These mesenchymal stem cells are isolated from umbilical cord blood (UCB) samples, harvested at birth from full-term deliveries. Cells exhibit a normal 46,XY karyotype.


Umbilical cord blood (UCB) samples are collected within 10 minutes of delivery, after removal of the placenta. UCB samples are subjected to Histopaque separation and the interface layer is then collected and centrifuged at 500g for 10 minutes. Cells are washed and further centrifuged at 350g for 5 minutes. UCB mononuclear cells (MNCs) are seeded in fibronectin-coated culture dishes, and cultured in Dulbecco’s modified Eagle medium (DMEM) high-glucose medium supplemented with fetal bovine serum (FBS, 10%), and penicillin-streptomycin (1%). Cells are incubated for 5-7 days in a humidified atmosphere containing 5% CO2, before cultured medium is replaced and non-adherent cells are removed.

See additional Stem, Progenitor & Primary Cells for: Blood , Mesenchymal Stem Cells , Umbilical Cord
Fetal Stem / Progenitor Cell
Homo sapiens
Umbilical cord blood-derived mesenchymal stem cells