IE users: please get the latest browser version at Microsoft Internet Explorer Home Page
if yot already have the latest browser version please verify that your browser settings is not in compatibility mode
These mesenchymal stem cells are isolated from umbilical cord blood (UCB) samples, harvested at birth from full-term deliveries. Cells exhibit a normal 46,XY karyotype.
Umbilical cord blood (UCB) samples are collected within 10 minutes of delivery, after removal of the placenta. UCB samples are subjected to Histopaque separation and the interface layer is then collected and centrifuged at 500g for 10 minutes. Cells are washed and further centrifuged at 350g for 5 minutes. UCB mononuclear cells (MNCs) are seeded in fibronectin-coated culture dishes, and cultured in Dulbecco’s modified Eagle medium (DMEM) high-glucose medium supplemented with fetal bovine serum (FBS, 10%), and penicillin-streptomycin (1%). Cells are incubated for 5-7 days in a humidified atmosphere containing 5% CO2, before cultured medium is replaced and non-adherent cells are removed.