Cord blood-derived hematopoietic stem cell (Arteriocyte)

CD34+ cells were isolated using an autoMACS® Pro Separator after immunomagnetic labeling with CD34 microbeads. CD34+ cells were either immediately seeded onto substrates or cryopreserved, thawed, and then seeded. Cells were seeded at a density of 1,000 cells/mL and cultured in Arteriocyte’s serum-free medium and growth factor cocktail.