IE users: please get the latest browser version at Microsoft Internet Explorer Home Page
if yot already have the latest browser version please verify that your browser settings is not in compatibility mode
Mesenchymal precursor cells (MPCs) are isolated from human bone marrow aspirates by density gradient separation over Ficoll for mononuclear cells followed by a STRO-3-based immunoselective magnetic activated cell sorting (MACS); cultures are then expanded. STRO-3 is a monoclonal antibody, which reacts with an epitope of human tissue non-specific alkaline phosphatase. Stro3 binds to the immunizing stromal cells, but lacks reactivity with peripheral blood-derived mononuclear cells.
Culture-expanded MPCs demonstrate increased gene expression of a range of stem cell markers relative to mesenchymal stem cells (MSCs), and exhibit a greater capacity to undergo osteogenic, adipogenic and chondrogenic differentiation.