Bone marrow-derived mesenchymal stem cells (Oslo University Hospital)

Cells are grown in medium consisting of: Dulbecco's Modified Eagle Medium (DMEM)-F12, penicillin/streptomycin (1%), amphotericin B (2.5 μg/mL), fetal bovine serum (10%). Medium is changed after 48 hours and then twice a week until colonies reach 70% confluence. After first passage, amphotericin B is no longer included in the culture medium.