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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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Welcome to
LifeMap Discovery

Watch the introductory video to learn more about our database of embryonic development for stem cell research and regenerative medicine

All in Vitro Cells > Adult Stem / Progenitor Cell card

Bone marrow-derived mesenchymal stem cells (Stempeucel™) (Stempeutics)

Ex vivo-cultured bone marrow-derived adult mesenchymal stem cells (Stempeucel). 


Culturing medium: Knock-out DMEM supplemented with fetal bovine serum (10%) and L-glutamine (2 mM) in T75 flasks. Harvested cells at the end of passage 0 (P0) are seeded at a density of 1000 cells/cm2 in two Cell STACK chambers. At 70–80% confluency (day 5–6), P(1) cells are harvested and an equal number of mesenchymal stem cells from each donor is pooled before plating them on a single CellSTACK, at a density of 1000 cells/cm2. Basic fibroblast growth factor (2 ng/mL) is added at P(2).

See additional Stem, Progenitor & Primary Cells for: Bone , Mesenchymal Stem Cells
Adult Stem / Progenitor Cell
Human
Bone marrow-derived mesenchymal stem cells (Stempeucel™)