Bone marrow-derived mesenchymal stromal cells (Soonchunhyang University Bucheon Hospital)

Bone marrow was isolated from the femur and the tibia of rats, flushed out using phosphate-buffered saline (PBS) containing fetal bovine serum (0.2%). Mesenchymal stem cells were recovered by their tendency to adhere tightly to plastic culture dishes. Filtered cells were plated in Dulbecco's modified Eagle's medium containing fetal calf serum (10%) and penicillin/streptomycin (100 U/mL and 0.1 mg/mL, respectively) and allowed to adhere for 24 h. Nonadherent cells were discarded. The culture medium was changed every 3 days.