Browser Not Supported.

IE users: please get the latest browser version at Microsoft Internet Explorer Home Page

if yot already have the latest browser version please verify that your browser settings is not in compatibility mode

Browser: unknown
Version: 0

EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
Content

Welcome to
LifeMap Discovery

Watch the introductory video to learn more about our database of embryonic development for stem cell research and regenerative medicine

All in Vitro Cells > Adult Stem / Progenitor Cell card

Bone marrow-derived lineage-negative cells (University of Sao Paulo)

Lin- cells are bone marrow derived lineage-negative cells that are negatively selected by several antibodies for lymphocytes, macrophages, granulocytes and erythrocytes.


Lin− cells are isolated from total bone marrow cells that are extracted from the femurs and tibias of rats and suspended in phosphate-buffered saline containing bovine serum albumin. Using magnetic beads, the cells are negatively selected with the following antibodies: anti-CD4 (0.125 μg/106 cells) and anti-CD8 (0.5 μg/106 cells) for T lymphocytes; anti-CD45RA (0.05 μg/106 cells) for B lymphocytes; anti-CD11b (0.5 μg/106 cells) for macrophages; anti-CD68 (0.125 μg/106 cells) for macrophages and granulocytes; and anti-erythrocyte (0.5 μg/106 cells) for red blood cells.

See additional Stem, Progenitor & Primary Cells for: Bone
Adult Stem / Progenitor Cell
Rat
Bone marrow-derived lineage-negative cells