IE users: please get the latest browser version at Microsoft Internet Explorer Home Page
if yot already have the latest browser version please verify that your browser settings is not in compatibility mode
Lin- cells are bone marrow derived lineage-negative cells that are negatively selected by several antibodies for lymphocytes, macrophages, granulocytes and erythrocytes.
Lin− cells are isolated from total bone marrow cells that are extracted from the femurs and tibias of rats and suspended in phosphate-buffered saline containing bovine serum albumin. Using magnetic beads, the cells are negatively selected with the following antibodies: anti-CD4 (0.125 μg/106 cells) and anti-CD8 (0.5 μg/106 cells) for T lymphocytes; anti-CD45RA (0.05 μg/106 cells) for B lymphocytes; anti-CD11b (0.5 μg/106 cells) for macrophages; anti-CD68 (0.125 μg/106 cells) for macrophages and granulocytes; and anti-erythrocyte (0.5 μg/106 cells) for red blood cells.