Bone marrow-derived mesenchymal stromal cells (FAMERP)

Bone marrow cells were isolated from femoral and tibial bones of rats and mononuclear elements were separated by Ficoll-Hypaque (d  1.077) density gradient centrifugation. Cells were cultured in DMEM supplemented with fetal bovine serum (20%) and penicillin/streptomycin/amphotericin, at a density of 1.5 x10⁶ mononuclear cells/cm², at 37°C with 5% CO₂.