This clone was derived from human dermal fibroblasts by viral integration. Lentivirus, ecotropic retrovirus, Transgenes: Oct4, Sox2, Klf4, c-Myc. Karyotype: Normal, 46XX.
Growth conditions: Generate and maintain iPS cells in primate ES medium (ReproCELL, Japan) supplemented with recombinant human basic fibroblast growth factor (bFGF, 4 ng/ml). Seed iPS cells on Matrigel-coated plates in MEF-conditioned primate ES cell medium, both supplemented with bFGF (4 ng/ml). Change the medium daily.
For preparation of MEF-conditioned medium: Plate MEFs derived from embryonic day 13.5 embryo pool of ICR mice at 1*10^6 cells per 100 mm dish and incubate overnight. Then, wash the cells once with PBS and culture in 10 ml primate ES cell medium. After 24 hr incubation, collect the supernatant of the MEF culture, filter through a 0.22 mm pore-size filter and store.