EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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Umbilical cord blood-derived endothelial progenitor cells (National Yang Ming University)

Endothelial progenitor cells were isolated from mononuclear cells of umbilical cord blood. When cultured in matrigel, cells formed tubular structures.


Blood mononuclear cells (MNCs) were isolated by Histopaque-1077 density-gradient centrifugation. MNCs were then cultured, on fibronectin-coated  plates, in endothelial growth medium-2, supplemented with hydrocortisone, IGF-1, human EGF, human VEGF, human FGF-B, ascorbic acid, GA-1000, heparin and 2% fetal bovine serum. Cells formed endothelial progenitor cell (EPC) colonies after 2-4 weeks of culturing.

See additional Stem, Progenitor & Primary Cells for: Endothelium , Umbilical Cord
Fetal Stem / Progenitor Cell
Homo sapiens
National Yang Ming University
Umbilical cord blood-derived endothelial progenitor cells
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