Browser Not Supported.

IE users: please get the latest browser version at Microsoft Internet Explorer Home Page

if yot already have the latest browser version please verify that your browser settings is not in compatibility mode

Browser: unknown
Version: 0

All in Vitro Cells > Fetal Stem / Progenitor Cell card

Epiblast Stem Cell line 7 (NINDS - National Institute of Health)

Epiblast stem cells were derived from the epiblast of post-implantation mouse embryos. Cells formed teratomas in-vivo, which contained cells of all the three germ layers. Cells have a normal karyotype (40,XX).

E5.5  mouse embryos were dissected out from their decidua layer, into HEPES-buffered medium. In order to isolate the epiblast layer, an incision was made at the boundary between the extra-embryonic ectoderm and the epiblast layers. The embryonic fragment consisting of the epiblast and the overlying visceral endoderm layer, was digested in a solution of  trypsin and  pancreatin in PBS and subsequently returned to the initial HEPES-buffered dissection medium. The isolated epiblast fragments were then cultured on a feeder cell layer in DMEM/F12, supplemented with  knockout serum replacement (20%),  FGF2 (5 ng/ml), 2-mercaptoethanol (0.1mM ),  L-glutamine (2mM), and  non-essential amino acids (X1). 

See additional Stem, Progenitor & Primary Cells for: Epiblast
Fetal Stem / Progenitor Cell
Mus musculus
Epiblast Stem Cell line 7