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Corneal limbal epithelial stem cells were harvested from cadavers and cultured on human lens capsule (LC) cells.
Limbal tissue was harvested from cadavers within 12 hours of biological death. The conjunctiva was incised and separated from the limbal junction. A small rectangular shape limbal graft was dissected away and towards the cornea. The graft was cultured on lens capsule cells in corneal epithelial cell culture medium, comprised of Dulbecco-modified Eagle’s medium (DMEM) supplemented with human AB serum (20%, human serum Type AB), L-glutamine ( 200 mM/mL), penicillin (10,000 U/mL), and streptomycin (10 mg/mL).