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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM

Line H7 (WA07) - Human Embryonic Stem Cell

This human ES cell line is positive for pluripotent markers and alkaline phosphatase and negative for mouse specific SSEA-1. In addition, it was shown to be positive for MHC class I antigens but negative for MHC class II. It has a normal 46, XX karyotype, B blood type.

WiCell Research Institute, Inc.
Inner Cell Mass, Early Embryo
Cultured cells on irradiated MEF feeder cells in ES medium comprised of KNOCKOUT–Dulbecco’s modified Eagle’s medium (KO-DMEM), KNOCKOUT serum replacement (20%), L-glutamine (1 mM), β-mercaptoethanol (0.1 mM), nonessential amino acids (1%), and human basic fibroblast growth factor (hbFGF, 4 ng/ml).
Version 1.1 Build 20