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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM

VUB03_DM1 - Human Embryonic Stem Cell

This line was derived from a preimplantation genetic diagnosis (PGD) embryo carrying mutations associated with myotonic dystrophy type 1 (DM1). DM1 is an autosomal dominant disorder caused by a mutation in the dystrophia myotonica protein kinase gene (DMPK). Cells are positive for pluripotent markers and have a 46,XX karyotype.

European Human Embryonic Stem Cell Registry
Inner Cell Mass, Early Embryo
Derivation and culture medium (hES medium): Knockout Dulbecco’s modified Eagle’s medium (KO DMEM) supplemented with Knockout-Serum Replacement (KO-SR; 20%), L-glutamine (2 mmol/l), non-essential amino acids (1%), beta-mercaptoethanol (0.1 mmol/l) and human recombinant basic fibroblast growth factor (hrbFGF; 4 ng/ml). CF1 MEF cells are used as feeder cells.
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