This line of human induced pluripotent stem cells (iPSCs) was generated by retroviral transduction of OCT3/4, KLF4, SOX2 and c-MYC genes into human dermal fibroblasts (HDFs). Normal karyotype 46XX.
The cells are maintained on SNL feeder cells (a mouse fibroblast STO cell line transformed with neomycin resistance and murine LIF genes) in primate ES medium supplemented with recombinant human basic fibroblast growth factor (bFGF; 4 ng/ml). The cells are passed using collagenase IV.