This induced pluripotent stem cell (iPSC) line was generated from CD34-positive bone marrow samples. Pluripotent cells were induced by a single EBNA1/OriP episomal plasmid expressing 5 reprogramming genes (OCT4, SOX2, KLF4, c-MYC and LIN28) as a single poly-cistronic unit. Cells express stem cell markers and display normal pluripotency, as determined by embryoid body (EB) formation and in vitro differentiation. Cells have a normal karyotype 46, XY.
Cells are maintained on a mitotically inactivated mouse embryonic fibroblast (MEF) feeder layer in knockout/DMEM medium, supplemented with knockout serum replacement (20%), L-glutamine (2 mM), nonessential amino acids (2 mM), antibiotic/antimycotic mix (X1), β-mercaptoethanol (0.1 mM), and basic fibroblast growth factor (10 ng/ml).