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All in Vitro Cells > Induced Pluripotent Stem Cell card

Bi.1-3 (University of Cambridge)

This induced pluripotent stem cell (iPSC) line was generated from adult dermal fibroblasts of a 28-year-old female.  The fibroblasts were infected with a mix of moloney murine leukemia virus–derived vectors, each containing the coding sequences of one of the following human genes: OCT4, SOX2, c-MYC, and KLF4Cells express pluripotency markers, and differentiate, in vitro, into cells from all the three germ layers.

For feeder- and serum-free culture, cells were grown in CDM, supplemented with activin (10 ng/ml) and bFGF (12 ng/ml). CDM medium: Iscove's modified Dulbecco's medium (50%) plus F12 NUT-MIX (50%), supplemented with insulin (7 μg/ml), transferrin (15 μg/ml), monothioglycerol (450 μM), and bovine serum albumin fraction V (5 mg/ml) or polyvinyl alcohol (CDM-PVA).

See additional Stem, Progenitor & Primary Cells for: Inner Cell Mass
Induced Pluripotent Stem Cell
Homo sapiens