This induced pluripotent stem cell (iPSC) line was generated from adult dermal fibroblasts of a 28-year-old female. The fibroblasts were infected with a mix of moloney murine leukemia virus–derived vectors, each containing the coding sequences of one of the following human genes: OCT4, SOX2, c-MYC, and KLF4. Cells express pluripotency markers, and differentiate, in vitro, into cells from all the three germ layers.
For feeder- and serum-free culture, cells were grown in CDM, supplemented with activin (10 ng/ml) and bFGF (12 ng/ml). CDM medium: Iscove's modified Dulbecco's medium (50%) plus F12 NUT-MIX (50%), supplemented with insulin (7 μg/ml), transferrin (15 μg/ml), monothioglycerol (450 μM), and bovine serum albumin fraction V (5 mg/ml) or polyvinyl alcohol (CDM-PVA).