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Cells were derived from inner cell mass (ICM) isolated from human blastocysts. FES22 cells form teratomas and differentiate into cells from the three germ layers. Cells have a normal karyotype 46,XY.
Cells were cultured on HFF or MEF feeder cells (mitotically inactivated) in serum-free medium consisting of Knockout-DMEM supplemented with L-glutamine ( 2 mM) penicillin/streptomycin, Knockout Serum Replacement (20% ), non-essential amino acids (X1), beta-mercaptoethanol (0.1 mM), ITS (X1), and bFGF (4 ng/ml).