HEL24.3 induced pluripotent stem cells were derived from human adult male foreskin fibroblasts using a non-integrating Sendai virus reprogramming kit. Reprogramming was conducted by expression of OCT4, SOX2, KLF4, and c-MYC. Cells express pluripotency markers and carry a normal karyotype:46,XY.
Cells are cultured on mitotically inactivated human foreskin fibroblast (hFF) feeder cells in Knock-Out Dulbecco’s Modified Eagle Medium (KO-DMEM) supplemented with Knock-Out Serum Replacement (20%), GlutaMax-I (2 mM), β-mercaptoethanol (0.1 mM), non-essential amino acids (NEAA, 1% ), penicillin/streptomycin (50 U/ml) and human bFGF (8 ng/ml).
EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
