HS360 (European Human Embryonic Stem Cell Registry)

The cells are cultured in Knockout Dulbecco's modified Eagle's medium  supplemented  with SR medium (20%),  L-glutamine (2 mM),  penicillin-streptomycin (1%),  nonessential amino acids (1%),  2-mercaptoethanol (0.5 mM), insulin-selenium-transferrin (1%), and bFGF (8 ng/ml).