This human embryonic stem cell line was derived by immunosurgical removal of the inner cell mass of human blastocysts. These cells are positive for pluripotent markers. Karyotype: 46,XY
The cells are cultured in Knockout Dulbecco's modified Eagle's medium supplemented with SR medium (20%), L-glutamine (2 mM), penicillin-streptomycin (1%), nonessential amino acids (1%), 2-mercaptoethanol (0.5 mM), insulin-selenium-transferrin (1%), and bFGF (8 ng/ml).