This induced pluripotent stem cell (iPSC) line was generated from dermal fibroblasts, by viral transduction of OCT4, SOX2, KLF4, c-MYC, hTERT, and SV40 Large T antigen transgenes. Cells express pluripotent markers and have a normal karyotype, 46XY.
Culture medium consists of DMEM/F12, supplemented with knockout serum replacement (20%), human recombinant basic fibroblast growth factor (10 ng/ml), nonessential amino acids (X1), 2-mercaptoethanol (5.5 mM), penicillin (50 units/ml), and streptomycin (50 μg/ml).