This cell line was derived from HES3 human embryonic stem cells, by targeting GFP-encoding sequences to the MIXL1 locus, using homologous recombination.
Cells were cultured on irradiated mouse embryonic fibroblast feeder cells in DMEM/F12 medium supplemented with KnockOut serum replacement (20%), MEM nonessential amino acids (100 μM), 2-mercaptoethanol (110 μM), penicillin/streptomycin (X1), Glutamax (X1) and bFGF (10 ng/ml).