This induced pluripotent stem cell (iPSC) line was generated from the human lung fibroblast line IMR90 that was transduced, using lentiviral vectors, with three reprogramming factors: OCT4, SOX2, and KLF4 . Cells express pluripotent markers and differentiate into all three germ layers, in both in-vitro and in-vivo settings. Cells have a normal karyotype, 46 XX.
Cells are cultured in KnockOut DMEM supplemented with KnockOut Serum (20%), nonessential amino acids (NEAA, X1), L-glutamine (X1), β-mercaptoethanol (0.1 mM), and basic fibroblast growth factor (10 ng/ml).