This human embryonic stem cell line was derived from the isolated inner cell mass of human blastocysts. These cells are positive for pluripotent markers. Karyotype: 46,XX
Cell are cultured on a human foreskin fibroblast (hFF) layer, in knockout-DMEM basal medium supplemented with knockout serum replacement (20%), Glutamax (2 mM), b-mercaptoethanol (0.1 mM), MEM non-essential amino acids (0.1 mM), penicillin-streptomycin (1%) and recombinant human bFGF (8 ng/ml).