Induced pluripotent stem cells (iPSCs) were generated from fibroblasts derived from a 2-month-old Caucasian male with clinical features of Crigler-Najjar syndrome, carrying a 13 base-pair deletion in exon 2 of UGT1A1 gene . The donated tissue was infected with a mix of moloney murine leukemia virus–derived vectors, each containing the coding sequences of one of the following human genes: OCT4, SOX2, c-MYC, and KLF4. Cells express pluripotency markers, demonstrate alkaline phosphatase activity, and form teratomas which contain cells from all the three germ layers.
Cells were cultured on irradiated mouse feeder cells in human embryonic stem cell culture medium (KSR) supplemented with bFGF (4 ng/ml).