Induced pluripotent stem cells (iPSCs) were generated from fibroblasts derived from a 2-month-old male with clinical features of hereditary tyrosinemia type 1, carrying a Val166Gly mutation in FAH gene (one allele). The donated tissue was infected with a mix of moloney murine leukemia virus-derived vectors, each containing the coding sequences of one of the following human genes: OCT4, SOX2, c-Myc, or KLF4. Cells express pluripotency markers, demonstrate alkaline phosphatase activity, and form teratomas, which contain cells from all the three germ layers.
Cells were cultured on irradiated mouse feeder cells in human ES cell culture medium (KSR) supplemented with bFGF (4 ng/ml).