Reprogrammed maturity onset diabetes of the young type 2 cells (Harvard Medical School)

Induced pluripotent stem cells (iPSCs) were maintained on inactivated  mouse embryonic fibroblasts (MEFs)  in Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 (DMEM/F12), supplemented with knockout serum replacement (KOSR, 20%), L-glutamine (2 mM), non-essential amino acids (NEAA, 10 mM), β-mercaptoethanol (50 mM), penicillin/ streptomycin (1X) and basic fibroblast growth factor (bFGF, 10 ng/ml).