IE users: please get the latest browser version at Microsoft Internet Explorer Home Page
if yot already have the latest browser version please verify that your browser settings is not in compatibility mode
This induced pluripotent stem cell (iPSC) lines was generated by transducing a lentivirus containing a novel combination of UTF1-, OCT-4-, SOX2- and KLF-encoding genes into human fibroblasts. iPSCs were generated more efficiently from human adult fibroblasts in the absence of the oncogene c-MYC.
Cells are maintained on mitomycin c-treated mouse embryonic fibroblasts in hES cell culture medium consisting of DMEM/F12, Knockout serum replacement (KSR; 20%), L-glutamine (1 mM), non-essential amino acids (1%), beta-mercaptoethanol (0.1 mM) and basic fibroblast growth factor (bFGF; 4 ng/ml). Cells are passaged using dispase II (Invitrogen) and medium is changed daily.