Browser Not Supported.

IE users: please get the latest browser version at Microsoft Internet Explorer Home Page

if yot already have the latest browser version please verify that your browser settings is not in compatibility mode

Browser: unknown
Version: 0

EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
Content
All in Vitro Cells > Embryonic Stem Cell card

VUB02 (European Human Embryonic Stem Cell Registry)

Line VUB02 was derived from IVF embryos at blastocyst stage. Isolated ICMs were transferred to 0.1% gelatin-coated culture dishes containing inactivated mouse embryonic fibroblast (MEF) feeder layers, and cultured further at 37°C in 10% CO2. Karyotype : 46,XY.


VUB02 was derived on inactivated MEFs isolated from 129/Sv mice. The derivation and Culture medium (hES medium): Knockout Dulbecco’s modified Eagle’s medium (KO DMEM) supplemented with fetal bovine serum (FBS, 20%), L-glutamine (2 mM/L), non-essential amino acids (1%), beta-mercaptoethanol (0.1 mmol/l), human recombinant leukaemia inhibitory factor (hrLIF, 103 U/ml) and human recombinant basic fibroblast growth factor (hrbFGF, 4 ng/ml). After a few passages, the culture conditions are changed by using CF1 MEF as feeder cells, omitting hrLIF and replacing FBS with Knockout-Serum Replacement (KO-SR).

See additional Stem, Progenitor & Primary Cells for: Inner Cell Mass
Embryonic Stem Cell
Homo sapiens
VUB02