IE users: please get the latest browser version at Microsoft Internet Explorer Home Page
if yot already have the latest browser version please verify that your browser settings is not in compatibility mode
This line was derived from a preimplantation genetic diagnosis (PGD) embryo carrying mutations associated with myotonic dystrophy type 1 (DM1), which is an autosomal dominant disorder caused by a mutation in the dystrophia myotonica protein kinase gene (DMPK). Cells are positive for pluripotent markers and have a 46,XX karyotype.
Derivation and culture medium (hES medium): Knockout Dulbecco’s modified Eagle’s medium (KO DMEM) supplemented with Knockout-Serum Replacement (KO-SR; 20%), L-glutamine (2 mmol/l), non-essential amino acids (1%), beta-mercaptoethanol (0.1 mmol/l) and human recombinant basic fibroblast growth factor (hrbFGF; 4 ng/ml). CF1 MEF cells are used as feeder cells.