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hESC were derived from human preimplantation embryos free of any known mutations associated with genetic diseases. The embryos were collected after IVF or preimplantation genetic diagnosis (PGD). Cells are positive for pluripotent markers and have a 46,XX karyotype.
Derivation and culture medium (hES medium): Knockout Dulbecco’s modified Eagle’s medium (KO DMEM) supplemented with Knockout-Serum Replacement (KO-SR; 20%) L-glutamine (2 mmol/l), non-essential amino acids (1%), beta-mercaptoethanol (0.1 mmol/l) and human recombinant basic fibroblast growth factor (hrbFGF; 4 ng/ml). CF1 MEF cells are used as feeder cells.