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hESC were derived from human preimplantation embryos after preimplantation genetic diagnosis (PGD). This line was characterized as carrying a mutation for facioscapulohumeral muscular dystrophy (FSHD). Cells are positive for pluripotent markers and have a 46,XX karyotype.
Derivation and culture medium (hES medium): Knockout Dulbecco’s modified Eagle’s medium (KO DMEM) supplemented with Knockout-Serum Replacement (KO-SR; 20%), L-glutamine (2 mmol/l), non-essential amino acids (1%), beta-mercaptoethanol (0.1 mmol/l) and human recombinant basic fibroblast growth factor (hrbFGF; 4 ng/ml). CF1 MEF cells are used as feeder cells.