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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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LifeMap Discovery

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All in Vitro Cells > Embryonic Stem Cell card

AS034 (European Human Embryonic Stem Cell Registry)

The AS034 cell line was derived from pronase-treated blastocysts and demonstrates alkaline phosphtase expression, detected via staining. The line was tested in vitro for formation of all three germ layers; it forms teratomas. Karyotype: 46,XY


Routinely passage every 4–5 days. hES cell colonies can be mechanically cut into pieces, 200 × 200 μm, removed from the culture dish and transferred to a new culture dish with fresh MEF cells and VitroHES supplemented with 4 ng/ml human recombinant bFGF (hrbFGF).
See additional Stem, Progenitor & Primary Cells for: Inner Cell Mass
Embryonic Stem Cell
Human
AS034