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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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LifeMap Discovery

Watch the introductory video to learn more about our database of embryonic development for stem cell research and regenerative medicine

All in Vitro Cells > Embryonic Stem Cell card

CyT203 (ViaCyte - (Novocell) San Diego, CA)

This cells were generated by ViaCyte.


Maintain undifferentiated hES cells on mouse embryo fibroblast feeder layers (Specialty Media) in DMEM/F12 supplemented with KnockOut serum replacement (20% (v/v)), nonessential amino acids (1 mM), Glutamax (x1), penicillin/streptomycin (x1), 2-mercaptoethanol (0.55 mM), recombinant human FGF2 (4 ng/ml) and activin A (10–25 ng/ml). Passage cells manually at a 1:4–1:8 split ratio, every 5–7 d.
See additional Stem, Progenitor & Primary Cells for: Inner Cell Mass
Embryonic Stem Cell
Human
CyT203