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This human embryonic stem cell line was derived from the isolated inner cell mass of human blastocysts. These cells are positive for pluripotent markers. Karyotype: 46,XY
Cells are cultured in medium consisting of knockout-DMEM supplemented
with knockout serum replacement (20%), GlutaMax (2 mM), MEM Eagle Non-Essential Amino acid solution (1%), penicillin/streptomycin
(0.5%), ß-mercaptoethanol (0.1 mM), and basic fibroblast growth factor (bFGF; 8 ng/ml).