Browser Not Supported.

IE users: please get the latest browser version at Microsoft Internet Explorer Home Page

if yot already have the latest browser version please verify that your browser settings is not in compatibility mode

Browser: unknown
Version: 0

EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
Content

Welcome to
LifeMap Discovery

Watch the introductory video to learn more about our database of embryonic development for stem cell research and regenerative medicine

All in Vitro Cells > Embryonic Stem Cell card

ES-J1 (ATCC)

The J1 ES line was derived from a male agouti 129S4/SvJae embryo. The J1 cells deposited at the ATCC were recloned from Dr. En Li's P6 J1 ES cells. The deposited line has been shown to be germline-competent.


Grow cells in HEPES-buffered (20 mM, pH 7.3) Dulbecco’s modified Eagle’s medium (DMEM) supplemented with fetal calf serum (15%), nonessential amino acids (0.1 mM), b-mercaptoethanol (0.1 mM), and antibiotics. Grow cells on feeder layers of y-irradiated embryonic fibroblast (EF) cells.
See additional Stem, Progenitor & Primary Cells for: Inner Cell Mass
Embryonic Stem Cell
Mouse
ES-J1