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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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All in Vitro Cells > Embryonic Stem Cell card

ES-J1 (ATCC)

The J1 ES line was derived from a male agouti 129S4/SvJae embryo. The J1 cells deposited at the ATCC were recloned from Dr. En Li's P6 J1 ES cells. The deposited line has been shown to be germline-competent.


Grow cells in HEPES-buffered (20 mM, pH 7.3) Dulbecco’s modified Eagle’s medium (DMEM) supplemented with fetal calf serum (15%), nonessential amino acids (0.1 mM), b-mercaptoethanol (0.1 mM), and antibiotics. Grow cells on feeder layers of y-irradiated embryonic fibroblast (EF) cells.
See additional Stem, Progenitor & Primary Cells for: Inner Cell Mass
Embryonic Stem Cell
Mus musculus
ES-J1