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Mesenchymal cells were isolated from the term decidua vera of human placenta.
Fetal membranes (amnions and chorions) with decidua vera were collected from vaginally delivered full-term fetal adnexa of healthy mothers and washed extensively. First, the amnion was separated from the area above the chorion. Second, the decidua vera was scraped from the chorionic membrane. The decidual tissue was then dissected into small pieces and was digested using collagenase. The resulting cell mixture was transferred through a nylon mesh and single-cell suspensions were seeded in cell culture plates and cultured in DMEM/F12 medium supplemented with fetal bovine serum (FBS, 10%), HEPES (15 mM), and antibiotics.