Placental mesenchyme-derived stem cells (MSCs) were isolated from the placenta tissue closest to the umbilical cord. This culture probably contains fetal and maternal cells. The seeded cells formed a carpet of fibroblast-like cells. After three passages, most of the cells stained positive for mesenchymal surface membrane markers.
Plate placental cell suspension on dishes coated with human placental fibronectin in Knockout DMEM containing fetal bovine serum (FBS; 10%), nonessential amino acids (1:100), beta-mercaptoethanol (55 μM), ciprofloxacin (10 μg/ml), and amphotericin B (10 μg/ml).