Umbilical cord-derived mesenchymal stem cells (302 Military Hospital of China)

The umbilical cord vessels were removed, and the mesenchymal tissue was diced into cubes, washed, and finally seeded into a T75-cm2 tissue culture flask. After 12–15 days of culture, the remnants of the cord fragments were removed, and the adherent cells were cultured until confluence (first passage). These UC-MSC were then cultured and collected between the third and fourth passages, for transfusion into patients.