Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) are isolated from the Wharton's jelly tissue of human umbilical cords. Cells have a fibroblast-like morphology and are spindle-shaped and elongated. Cells express the mesenchymal antigens but are negative for hematopoietic markers.
Wharton's jelly mesenchymal stem cells (WJ-MSCs) were derived from Wharton's jelly tissue of human umbilical cords (UCs) processed within 6 hours of birth. WJ cells were treated with collagenase IV (2 mg/ml) for 16 hours and then with trypsin (2.5%) for 30 minutes, and then plated and cultured in complete human mesenchymal stem cell growth medium (hMSCGM).