Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) were isolated from the Wharton's jelly of human umbilical cords. WJ-MSCs have a fibroblastoid-shaped morphology. They express mesenchymal antigens but are negative for hematopoietic markers. Cells also express proliferation genes, neurogenesis genes and several microRNAs (miRNA). WJ-MSCs can undergo osteogenic and adipogenic differentiation.
Wharton's jelly mesenchymal stem cells (WJ-MSCs) were derived from Wharton's jelly (WJ) of human umbilical cords (UCs) processed within 24 hours of collection time. WJ cells were digested with collagenase and trypsin, washed twice in phosphate-buffered saline (PBS), plated and cultured in MesenCultTM medium. Nonadherent cells were removed the next day by medium exchange. The remaining adherent fibroblast-like cells were passaged after 4-7 days.